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Project 1: Investigating the role of FAM198b in angiogenesis, tumourgenesis and wound repair and Project 2: Isolation and culture of mesenchymal stem cells for craniofacial tissue regeneration

Sample, Klarke Michael (2011)
M.Res. thesis, University of Birmingham.

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Project 1 Abstract:
The short project detailed in this paper explores the role of Family with sequence similarity 198, member B (FAM198b) in angiogenesis. Angiogenesis is a complex process where many genes are involved in regulating the process of new blood vessel growth. Angiogenesis is a natural component in wound healing, but can be hijacked and contribute to tumourgenesis. Anti-angiogenic drugs have been proven to increase the survival probability of cancer patients with solid tumours. The current anti-angiogenic drugs have been targeted to proteins involved in the vascular endothelial growth factor (VEGF) signalling pathway. However tumours in many cancer patients have developed resistance to these therapies. For this reason alternative targets and therapeutic agents are needed to reduce the exploitation of alterative pro-angiogenic pathways in cancer patients. The potential of FAM198b to be used as a target for anti-angiogenic therapeutics has been discussed in this paper based upon the results of in vitro angiogenesis assays. These assays are designed to assess the effect of a FAM198b knockdown on the ability of human umbilical cord endothelial cells (HUVECs) to migrate, proliferate and form tubes.

Project 2 Abstract:
This project forms the basis of further work to investigate whether changes to the extracellular matrix can stimulate the differentiation of bone marrow derived stem cells along specific lineages for the purposes of craniofacial repair. Corrective surgery can be conducted, with varying degrees of success, to repair craniofacial tissues. This often requires removing tissue from other sites within the body, however a significant amount of tissue is required and obtaining sufficient donor material is not always possible. Tissue engineering could potentially provide viable alternative to transplantation. The implanted tissues could be constructed using synthetic or organic materials which can be grown after implantation into the required functionality. Alternatively these tissues could be engineered in vitro to form functional organs or tissues prior to implantation. Collagen gels are particularly suitable for the purpose of craniofacial regeneration not only because of their physical properties. Collagen gels are biocompatibility and have low immunogenicity properties. Furthermore collagen gels can be created to imitate the structure of many tissue types and is capable of maintaining its structure without deforming or collapsing.

Type of Work:M.Res. thesis.
Supervisor(s):Bicknell, Roy and Heath, Victoria L and Shelton, Richard M. and Scheven, Ben and Cooper, Paul
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Immunity and Infection
Subjects:Q Science (General)
R Medicine (General)
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
RK Dentistry
RZ Other systems of medicine
Institution:University of Birmingham
ID Code:3038
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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