Barnes, Amy (2011)
M.Res. thesis, University of Birmingham.
Virus entry into target cells is mediated by the attachment of viral glycoproteins to cell surface receptors. Entry of the hepatitis C virus (HCV) into hepatocytes has been shown to require viral glycoprotein dimer E1E2 and the target cell receptor CD81, scavenger receptor-BI (SR-BI) and the tight junction proteins claudin-1 and occludin in an undefined mechanism. In this study, we aim to define the dynamic movements of these proteins in the membrane of the polarising hepatocarcinoma cell line HepG2. We further examine the extent to which these kinetics are perturbed following addition of the soluble viral glycoproteins sE2 and sE1E2, and the small molecule inhibitor of SR-BI ITX5061.
The results of this study show that the amount of mobile receptor and the speed at which it diffuses varies according to its location within the cell. CD81 and claudin-1 are expressed equally in the filopodia and plasma membrane, whereas SR-BI is expressed at lower levels in the filopodia compared to the plasma membrane. We show that addition of both sE2 and sE1E2 has varying affects on both the speed and mobility of CD81 and claudin-1 and that the majority of significant effects observed for claudin-1 are observed at areas of potential cell contact. Finally, we demonstrate that addition of ITX5061 affects the diffusion coefficient of CD81 and CLDN-1 and the amount of mobile SR-BI. Furthermore, the effects on SR-BI are limited to areas of cell contact or exploratory regions. In summary, we present data which we hope will further current knowledge of the activity of these receptors in relation to their role in HCV infection.
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