Cook, Sarah Louise (2011)
M.Res. thesis, University of Birmingham.
CC-Chemokine receptor like 2 (CCRL2) is the newest member of the atypical chemokine receptor family, a set of proteins which tend to act as “decoy receptors”, causing a chemoattractant gradient of their ligands. Using SYBR-Green and Taqman RT-PCR analysis of murine tissue, this study aimed to characterise the expression of CCRL2 in the spleen, thymus and lymph nodes. Using specifically designed primers, the PCR techniques detected an alternative, “long”, spliced variant of CCRL2 within the thymus. CCRL2 mRNA expression was also defined over 8 days, during the lifetime of the germinal centre. CCRL2 mRNA expression was measured within B cells, the germinal centre or the T Zone. Due to its high expression within plasma B cells at days 4 and 7, CCRL2 may be involved in plasma cell exit from the GC. However, CCRL2 mRNA was also expressed within the germinal centre on days 7 and 8, which may suggest the receptor also has a role in germinal centre breakdown. Finally, this study also started the process of monoclonal antibody production to murine CCRL2. CCRL2 DNA was successfully cloned into a plasmid vector and transformed into E. coli in preparation for transfection into a mammalian cell line
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