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Biological effects of low frequency ultrasound on bone and tooth cells

Man, Jennifer Sui-Sum (2011)
Ph.D. thesis, University of Birmingham.

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Little is known about the biological effects of ultrasound on dental-derived cells and whether ultrasound may be used as a therapeutic tool in dental care. This thesis has investigated the functional responses of in vitro osteoblast and odontoblast model cell lines to low frequency ultrasound as a potential tool for dental tissue repair. Two methods for ultrasound delivery were used to stimulate cells in vitro; a dental ultrasonic scaler (EMS) capable of emitting ultrasound at a frequency of 30kHz; and the DuoSon (SRA developments) therapeutic ultrasound exposure system, which allowed the comparison of kHz, MHz and a combined frequency ultrasound. Odontoblast-like cells positively responded to all ultrasound frequencies applied and can increase VEGF expression, increase cell number and increase mineral deposition by enhancing differentiation when compared with sham-treated control. Furthermore, enhanced wound healing by increased cell migration and cell proliferation was demonstrated in ultrasound-stimulated osteoblast-like cells. Ultrasound induced a dose-dependent response in β-catenin staining in both odontoblast and osteoblast model cell lines, which implicates the Wnt/β-catenin pathway as a possible mechanism for intracellular ultrasound transduction. Taken together, it is tempting to speculate that direct low frequency ultrasound stimulation of the dentine-pulp complex or alveolar bone may be able to initiate or enhance regenerative events

Type of Work:Ph.D. thesis.
Supervisor(s):Cooper, Paul and Shelton, Richard M. and Scheven, Ben
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Dentistry
Subjects:RK Dentistry
Institution:University of Birmingham
ID Code:2878
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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