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Bacterial biofilms and biomineralisation on titanium

Wang, Anqi (2011)
Ph.D. thesis, University of Birmingham.

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Abstract

This study investigated bacterial interactions with titanium, and evaluated the use of Serratia biomineralisation to produce a hydroxyapatite (HA) coating on titanium. Adherence of Gram-positive Staphylococcus epidermidis and Streptococcus sanguinis and Gram-negative Serratia sp. NCIMB 40259 and Escherichia coli was compared on commercially pure titanium, Ti6Al4V alloy, pure aluminium and pure vanadium. Grain boundaries, grain orientation and alloy phase structure did not influence adhesion or early proliferation. Adherence of all four strains was equivalent on pure titanium and Ti6Al4V and inhibited on pure aluminium. Serratia biomineralisation was used to introduce a crystalline coating on Al\(_2\)O\(_3\) grit blasted titanium discs and a porous titanium mesh. The porous coating consisted of micro-scale spheres composed of nano-scale calcium deficient HA. Embedded alumina particles and alkali treatment did not noticeably alter precipitation of Serratia HA, nor the structure of the coating in comparison with non-treated substrates. Coatings were retained after sintering at 800\(^\circ\)C in argon, although the original curved plate-like crystals changed to nano-scale β-tricalcium phosphate particles. A phosphorous-rich diffusion zone formed at the coating-titanium interface. This biomineralised coating may have applications for coatings of implants in non load-bearing sites, and other non-clinical applications where a high surface area is the major concern.

Type of Work:Ph.D. thesis.
Supervisor(s):Jones, Ian P. and Sammons, Rachel L. and Mei, Junfa
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Metallurgy and Materials
Subjects:TA Engineering (General). Civil engineering (General)
QR Microbiology
Institution:University of Birmingham
Copyright Holders:Anqi Wang, Ian P Jones, Rachel L Sammons, Junfa Mei
ID Code:1562
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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