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Metallo-supramolecular cylinders and their peptide conjugates. Synthesis, dynamics and DNA recognition

Cardo, Lucia (2010)
Ph.D. thesis, University of Birmingham.

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Abstract

The work described in this thesis focuses on two main topics: i) the study of the dynamic behaviour of metallo-supramolecular cylinders, that are known for their major groove DNA binding properties, and ii) the synthesis and DNA binding studies of the cylinders conjugated to short peptides and amino acids. In Chapter 1 an overview of the DNA molecular recognition by synthetic agents and biomolecules is presented, with a section dedicated to the selective recognition of DNA. In Chapter 2 the study of the dynamic behaviour of the Fe(II) supramolecular cylinder is explored. The behaviour of the complex at variable temperature conditions was analysed by NMR, UV-Vis and CD. Thermal experiments with the complex in the presence of DNA are also presented. In Chapter 3 a versatile procedure for the conjugation of short amino acids and peptides to Fe(II), Cu(I) and Ag(I) cylinders has been established and the influence of the attached peptides on the chirality of the resulting hybrids has been investigated. In Chapter 4 the DNA binding properties of the conjugated cylinders are explored by CD, LD and gel electrophoresis experiments and the cytotoxic activity of the Fe(II) conjugates against cancer cell lines was investigated by MTT tests.

Type of Work:Ph.D. thesis.
School/Faculty:Colleges (2008 onwards) > College of Engineering & Physical Sciences
Department:School of Chemistry
Subjects:QD Chemistry
Institution:University of Birmingham
ID Code:1251
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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