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Neutrophil extracellular traps in periodontitis

Palmer, Lisa Joanne (2010)
Ph.D. thesis, University of Birmingham.

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Abstract

This thesis has investigated the role of neutrophil extracellular traps (NETs) in the pathogenesis of periodontitis. A fluorometric assay was developed for the in vitro quantification of NET release and used to assess relative levels in chronic periodontitis patients, indicating elevated release under several stimulatory conditions compared with controls. Studies also demonstrated that challenge with periodontitis-associated bacteria evoked differential NET release. In addition, an extensive panel of periodontal pathogens was screened for two potential virulence traits that may confer a survival advantage when challenged with NETs. Deoxyribonuclease (DNase) secretion, capable of degrading NETs, and resistance to killing by histone, a core structural and antimicrobial protein within NETs, were both shown to be relatively common characteristics possessed by pathogens associated with severe disease. Additionally, an in vivo study demonstrated DNase activity in plaque and GCF during the development of experimental gingivitis. Further studies examined the association between neutrophil reactive oxygen species production and NET release implicating a regulatory role for myeloperoxidase generated hypochlorous acid in NET production. In conclusion, the results contained within this thesis indicate a putative role for NETs in the pathogenesis of periodontitis and highlight a high prevalence of potential counter-NET mechanisms in certain periodontal organisms.

Type of Work:Ph.D. thesis.
School/Faculty:Colleges (2008 onwards) > College of Medical & Dental Sciences
Department:School of Dentistry
Subjects:RK Dentistry
Institution:University of Birmingham
Library Catalogue:Check for printed version of this thesis
ID Code:1199
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
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