eTheses Repository

# Oral epithelium in the pathogenesis of periodontitis

Milward, Michael Robert (2010)
Ph.D. thesis, University of Birmingham.

 PDF (4036Kb)

## Abstract

Pocket/sulcular epithelium is the first line of defence to plaque bacteria and its potential role in periodontitis is investigated. This thesis describes the development of a model system, utilising an immortal epithelial cell line (H400) in order to investigate responses to periodontal pathogen stimulation (P. gingivalis and F. nucleatum) in terms of NF-$$\kappa$$B activation, differential gene expression and cytokine production. The pathogenesis of periodontitis suggests that susceptible patients exhibit a hyper-inflammatory response to plaque bacteria, so an attempt to modulate the pro-inflammatory epithelial response using a natural di-thiol antioxidant $$\alpha$$-lipoate was also investigated. Data demonstrated that periodontal pathogens P. gingivalis and F. nucleatum elicited a pro-inflammatory response in the H400 model system. This was confirmed by demonstrating NF-$$\kappa$$B activation, gene expression changes and downstream cytokine production. Ontological grouping of gene expression changes revealed a range of gene functions which support the hypothesis that the epithelium may play a role in the initiation and propagation of the periodontal lesion. In addition, alipoate was able to modulate this inflammatory response but not completely block this essential defence mechanism. Data obtained indicates the potential of utilising $$\alpha$$-lipoate as an adjunct in the management of periodontitis.

Type of Work: Ph.D. thesis. Chapple, Iain L. (Iain Leslie) and Matthews, John and Cooper, Paul Colleges (2008 onwards) > College of Medical & Dental Sciences School of Dentistry, Unit of Periodontology RK Dentistry University of Birmingham 1007
This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.

Repository Staff Only: item control page