Download Statistics
Download StatisticsHajiyavand, Amir Mohammad (2018). Toward autonomous cell recognition and manipulation for intra-cytoplasmic sperm injection. University of Birmingham. Ph.D.
|
Hajiyavand2018PhD.pdf
Text - Accepted Version Available under License All rights reserved. Download (5MB) | Preview |
Abstract
Micromanipulation and microinjection of biological cells are two of the most useful techniques for manipulating cells for different applications. One of these applications, employed for infertility treatments, is intra-cytoplasmic sperm injection. The current setup for this application is designed to be operated manually by an embryologist. In this method, a single sperm is deposited into an oocyte. There are a number of non-clinical factors affecting the success rate of fertility. These factors can cause cells to be damaged at the time of injection and resulting in its degeneration after sperm deployment. This thesis focuses on analysing these factors, in particular the creation of deformation of the cell at the time of injection. The speed of injection has been considered as critical factors linked to the creation of oocyte deformation during injection. The main aim of this research is to develop an autonomous cell manipulation and injection system whilst minimising cell deformation during injection. A proposed set-up is designed to manipulate a single oocyte in three dimensional space to the desired location using a minimal number of operations. After the operation, the injection of sperm proceeds and is delivered into the desired position inside the oocyte. New software has been designed for the polar body and oocyte position and orientation recognition. Furthermore, an FE model has been developed to help the analysis of different factors affecting the injection process and provides information on the injection force and deformation creation during the injection.
| Type of Work: | Thesis (Doctorates > Ph.D.) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Award Type: | Doctorates > Ph.D. | |||||||||
| Supervisor(s): |
|
|||||||||
| Licence: | All rights reserved | |||||||||
| College/Faculty: | Colleges > College of Engineering & Physical Sciences | |||||||||
| School or Department: | School of Engineering, Department of Mechanical Engineering | |||||||||
| Funders: | None/not applicable | |||||||||
| URI: | http://etheses.bham.ac.uk/id/eprint/9997 |
Actions
 |
Request a Correction |
 |
View Item |
Downloads
Downloads per month over past year