Download Statistics
Download StatisticsPillay, Kumar Sanjana (2019). Characterisation of antisense non-coding RNAs during early zebrafish development. University of Birmingham. Ph.D.
|
Pillay2019PhD.pdf
Text - Accepted Version Available under License All rights reserved. Download (27MB) | Preview |
Abstract
Long non-coding RNA’s are a broad class of non-protein coding RNAs >200 nucleotide in length. Although the importance of lncRNAs in various cellular functions is increasingly becoming clear, their role in regulating vertebrate development still remains unexplored. A recent study on gene expression during zebrafish development identified 1133 lncRNAs, a majority of these lncRNAs (566) were antisense ncRNA (Pauli et al., 2012). Antisense lncRNAs, a class of lncRNAs, have been shown to act either as positive or negative regulators of overlapping protein-coding mRNAs.
To understand the role of antisense RNAs in early vertebrate development, we took a data mining approach. A computational analysis of RNA sequencing data from zebrafish development indicates that a number of antisense RNAs are present in embryos even before the zygotic gene transcription is initiated indicating that they are probably of maternal origin. In addition, our results hint at the role of the antisense RNAs in regulating transcription of developmental genes. Additionally, implying their role in zygotic genome activation. To further investigate this hypothesis, we employed transcriptomics methods to examine RNAs from the nuclear and cytosolic fractions from zebrafish embryos at different stages of development. The RNA-seq and CAGE-seq revealed spatial and temporal regulation of lncRNA expression during zebrafish development. It further revealed differences in promoter width and TSS usage between the nuclear and cytosolic fractions.
| Type of Work: | Thesis (Doctorates > Ph.D.) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Award Type: | Doctorates > Ph.D. | |||||||||
| Supervisor(s): |
|
|||||||||
| Licence: | All rights reserved | |||||||||
| College/Faculty: | Colleges > College of Life & Environmental Sciences | |||||||||
| School or Department: | School of Biosciences | |||||||||
| Funders: | European Commission | |||||||||
| Subjects: | Q Science > Q Science (General) | |||||||||
| URI: | http://etheses.bham.ac.uk/id/eprint/9711 |
Actions
 |
Request a Correction |
 |
View Item |
Downloads
Downloads per month over past year