A gellan-based fluid gel carrier for spray delivery

ter Horst, Britt (2019). A gellan-based fluid gel carrier for spray delivery. University of Birmingham. Ph.D.

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Abstract

Autologous cell transplantation is a promising approach to enhance burn wound reepithelialisation. It was introduced to clinical practice decades ago with current delivery techniques involving spraying autologous cultured or uncultured cells in low-viscosity suspensions. This delivery method is limited since it results in an uneven distribution on the wound bed and cell loss as the liquid is not retained on the skin surface. In this thesis, a sprayable gel that solidifies on the surface of the skin has been developed to circumvent this problem. A gellan-based fluid gel system was developed with flexible viscoelastic properties that can be tuned by a biocompatible polymer concentration and ionic strength to facilitate spray delivery. The material liquefies at high shear during spraying with self-healing properties of the gel causing it to solidify on the receiving surface. Results present the development and characterisation of a gellan fluid gel for the encapsulation and spray-delivery of cells onto surfaces, aiming for reduced cell spillage, improved spray uniformity and maintenance of cell viability and function. Additionally, the same polymer was used to formulate a gellan film that can be rehydrated in acetic acid for the treatment of wound infections. Gellan is a versatile polymer as shown by the formulations explored in this study and a promising candidate for cell and drug delivery applications.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):