Assessment of the toxicity of lipid nanocapsules and polymer nanoparticles on a neuronal cell model as a first step towards their development for nose-to-brain drug delivery

Utami, Rifka Nurul (2019). Assessment of the toxicity of lipid nanocapsules and polymer nanoparticles on a neuronal cell model as a first step towards their development for nose-to-brain drug delivery. University of Birmingham. M.Sc.

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Abstract

Nose-to-brain delivery is a promising alternative to deliver therapeutics to the central nervous system, due to the possibility to bypass the blood-brain barrier. Because of their versatility, nanoparticulate delivery systems may offer several advantages for this route. This study investigates the correlation between physicochemical characteristics of two different types of nanomaterials, namely lipid nanocapsules (LNCs) and PLGA nanoparticles (PLGA-NPs), and their behaviour towards SH-SY5Y cells as the neuronal cell model. Physicochemical characterisation and stability studies showed that all the formulations exhibited excellent properties and stability under storage (4 oC) and physiological conditions. Surface hydrophobicity was also assessed wherein PLGA-NPs were found to be generally more hydrophobic compared to LNCs. Cytotoxicity assays towards SH-SY5Y cells suggested that surfactant-related toxicity is more likely to occur in LNCs compared to PLGA-NPs. Cellular uptake was also analysed using flow cytometry and fluorescence microscopy. The results from this study can be used as consideration in developing nanoparticulate delivery system for nose-to-brain delivery.

Type of Work:

Thesis (Masters by Research > M.Sc.)

Award Type:

Masters by Research > M.Sc.

Supervisor(s):