A self-setting gel system for kartogenin delivery and retention in cartilage defects

Hodgkins, Jodie (2018). A self-setting gel system for kartogenin delivery and retention in cartilage defects. University of Birmingham. M.Phil.

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Abstract

A substantial proportion of the UK population are afflicted by cartilage damage every year, as a consequence of sporting injury or osteoarthritis. Current treatment therapies available are inadequate at providing a lasting result, while developing cell-based therapies present significant costs, resulting from strict regulations surrounding manufacturing processes. An economical solution was sought through development of an injectable bedside technology in the form of an alginate fluid gel-microenvironment and scaffold, capable of sustaining and delivering cells with chondrogenic potential, as well as retaining them during the healing process. Initial work investigated the effect of polymer concentration, with 2% gels demonstrating greater retention within model defects and superior self-setting ability. Subsequent analysis of the fluid gel microstructure revealed alignment of strands or fibres within the gel network that may influence its mechanical properties. Successful incorporation of MC3T3 fibroblast cells within alginate fluid gels was achieved, with the majority of cells expressing excellent viability over a 7-day culture period. Similar results were seen following metabolic activity analysis of BMSCs and human BMAC cells over 14-day and 21-day culture periods. Release profile studies also demonstrated steady release of KGN from the fluid gel matrix over 7 days, with no evidence of immediate burst release.

Type of Work:

Thesis (Masters by Research > M.Phil.)

Award Type:

Masters by Research > M.Phil.

Supervisor(s):