Role of long non-coding RNA \(CCDC26\) in gene regulation

Wijesinghe, Susanne (2018). Role of long non-coding RNA \(CCDC26\) in gene regulation. University of Birmingham. Ph.D.

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Abstract

LncRNAs are increasingly being recognised as functionally important for regulation of biological processes. We have identified an lncRNA which we believe is integral in lineage commitment during haematopoiesis. Here, we report that \(CCDC26\) lncRNA is a regulator of β-globin and c-MYC gene expression. Indeed, \(CCDC26\) silencing in erythroleukemic K562 cells led to several gene expression changes. Upon further investigation of genes linked to erythropoiesis, we observed the upregulation of β-globin expression. Our results suggest that \(CCDC26\) regulates expression of β-globin and other genes by modulating epigenetic changes which could be important in linage-commitment. We have results to suggest the expression of β-globin and c-MYC genes may be synergistic and promotes differentiation of K562. Finally, RNAseq data further supports upregulation of differentiation specific genes further underpinning our hypothesis that this lncRNA may play an important role in lineage commitment.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):