Dried blood spot analysis in routine clinical practice

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Shea, Robyn Lisa (2017). Dried blood spot analysis in routine clinical practice. University of Birmingham. Ph.D.

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Abstract

Dried blood spots (DBS) are drops of capillary blood collected onto filter paper from a finger prick. They have many advantages compared with traditional phlebotomy and enable patients to take samples at home. A DBS collection device was developed and incorporated into a CE marked DBS collection kit. This was successfully used in an international direct access vitamin D DBS service. A random access DBS CRP method was established for use with the DBS collection device and a new microsampling device called the Mitra. The quality of DBS received and the impact of lancet type was assessed and the effect of blood spot characteristics on CRP and vitamin D concentration was examined. The vitamin D service uptake and the population using it was analysed. The vitamin D concentration and status of users was compared to serum
samples received in the laboratory from the local GP population. Significant differences between the populations were seen, with DBS users showing higher levels of vitamin D. In addition, the response to vitamin D testing for both populations was analysed. A higher rate of high to toxic vitamin D levels was seen in the blood spot population and the reasons for this were explored.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

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