Effects of low-level light therapy on epithelial progenitor cells

Zainal, Siti Aishah (2017). Effects of low-level light therapy on epithelial progenitor cells. University of Birmingham. Ph.D.

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Abstract

Low level light therapy has been widely used in the management of a range of human diseases. Light irradiation triggers a range of cellular signalling processes in a variety of cells, promoting wound healing and preventing cell death. The aim of this study was to investigate the photobiomodulatory effects of low level lasers and light-emitting diodes (LEDs) on human oral epithelial cells (H400 cells) as well as neutrophils, as a potential management strategy for periodontitis. Initially light sources were characterised to obtain dosage (radiant exposure) for light experiments. In addition, a model system utilising H400 cells was developed and characterised prior to laser and LEDs irradiation analysis. Biological responses were determined upon irradiation.

Results demonstrated that irradiation by laser and LEDs enhanced H400 cell growth. This was described by mitochondrial metabolic activity and cell proliferation marker, Ki-67. This supports the ability of low-level light to trigger cell growth for further healing inflammation in periodontal disease. Furthermore, ROS production by human neutrophils was attenuated following LEDs irradiation and this suggests this light therapy may decrease level of neutrophil ROS in inflamed tissue and improve wound healing.

Data suggested potential therapeutic benefits for enhancing healing in the gingival epithelium, which propose the possibility of the use of light therapy, a non-invasive tool in periodontal disease management.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

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