The scale up of air-filled emulsion production

Critchley, Liam John (2016). The scale up of air-filled emulsion production. University of Birmingham. M.Sc.

[img]
Preview
Critchley16MSc.pdf
PDF - Accepted Version

Download (5MB)

Abstract

An Air-Filled Emulsion (AFE) is a dispersion of particles, of colloidal size, which are generally characterised from sub-micron to ten microns in diameter. They are produced by unfolding proteins so that the disulphide bonds in the protein react with superoxide radicals to form ‘air cells’. These air cells are what form the non-aqueous phase in AFEs. The aim of this project was to see if the production of AFEs could be scaled up from bench to pilot scale. Results showed that recycle methods produced up to 98.7 % AFE yield with two out of three top-up methods (STUM) producing >90 % AFE yield. Other methods which were non-top-up (NoTUM) did not produce a high yield, but showed that air cells are robust enough to withstand multiple passes of sonication waves and heating. These methods yielded the first continuous method of AFE production. This was adapted to produce a novel way of isolating and concentrating formulation-ready AFE, which was concentrated up to 6-times previously found using a cross-flow filtration module. Work on the pilot scale produced the first AFE sample beyond the bench scale, and combined with the novel methods it provides a promising benchmark to take the production to larger scales in the future.

Type of Work: Thesis (Masters by Research > M.Sc.)
Award Type: Masters by Research > M.Sc.
Supervisor(s):
Supervisor(s)EmailORCID
Simmons, Mark J. H.UNSPECIFIEDUNSPECIFIED
Cox, Philip WilliamUNSPECIFIEDUNSPECIFIED
Licence:
College/Faculty: Colleges (2008 onwards) > College of Life & Environmental Sciences
School or Department: School of Biosciences
Funders: Other
Other Funders: Innovate UK
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
URI: http://etheses.bham.ac.uk/id/eprint/7107

Actions

Request a Correction Request a Correction
View Item View Item

Downloads

Downloads per month over past year