Cronin, Laura (2016). The chronic lymphocytic leukaemia lymph node microenvironment. University of Birmingham. Ph.D.
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Cronin16PhD.pdf
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Abstract
The lymph node (LN) microenvironment in Chronic Lymphocytic Leukaemia (CLL) is the main site of disease progression and maintenance. Whilst isolated components of the LN niche have been studied \(in\) \(vitro\), to date, no comprehensive architectural overview of the microenvironment has been attempted. A more holistic view is essential in order to fully understand this disease. LN CLL cells are likely to receive a complex array of survival signals from accessory cells which drive disease and protect against conventional therapeutics. This study embarked upon establishing reliable combinations of primary and secondary antibodies that permit multicolour immunohistochemistry (IHC) interrogation of the CLL LN in formalin fixed paraffin embedded samples (FFPE). This work serves to demonstrate that the architecture of the CLL microenvironment is complex, dynamic and heterogeneous and highlights the advantages multicolour IHC can present to the field for understanding the therapeutic opportunities in this disease.
| Type of Work: | Thesis (Doctorates > Ph.D.) | |||||||||
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| Award Type: | Doctorates > Ph.D. | |||||||||
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| College/Faculty: | Colleges (2008 onwards) > College of Life & Environmental Sciences | |||||||||
| School or Department: | School of Biosciences | |||||||||
| Funders: | None/not applicable | |||||||||
| Subjects: | Q Science > QR Microbiology | |||||||||
| URI: | http://etheses.bham.ac.uk/id/eprint/6787 |
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