The identification of non-protein coding rna in the tumour vasculature

Sample, Klarke Michael (2016). The identification of non-protein coding rna in the tumour vasculature. University of Birmingham. Ph.D.

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Abstract

In the last 15 years a number of studies have sought to identify markers of tumour endothelium. However, studies have yet to identify non-protein markers of the tumour vasculature. This thesis provides novel contributions in this area by using RNA sequencing (RNAseq) to probe the transcriptome of tumour endothelial cells (TEC) and healthy tissue associated endothelial cells (HEC). The ensuing bioinformatic analysis revealed many differentially expressed short nuclear RNA (snoRNA) and long intergenic ncRNA (lincRNA) molecules. Some of which were confirmed as being specifically expressed in (and differentially expressed between) TEC and HEC using quantitative PCR (qPCR). One of these molecules was also functionally explored in vitro and is the first ncRNA molecule to be identified as both specific to the endothelium and to be functional in pathological angiogenesis.

Type of Work:	Thesis (Doctorates > Ph.D.)
Award Type:	Doctorates > Ph.D.
Supervisor(s):	Supervisor(s) Email ORCID Bicknell, Roy UNSPECIFIED UNSPECIFIED Heath, Victoria L UNSPECIFIED UNSPECIFIED
Licence:
College/Faculty:	Colleges (2008 onwards) > College of Medical & Dental Sciences
School or Department:	School of Immunity and Infection
Funders:	None/not applicable
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
URI:	http://etheses.bham.ac.uk/id/eprint/6696

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