Anthracene tagged biomolecules for DNA binding

Bullen, Gemma Anne (2015). Anthracene tagged biomolecules for DNA binding. University of Birmingham. Ph.D.

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Abstract

Within this thesis, the use of anthracene to perform various applications within biomolecules is assessed. Anthracene displays two interesting photo properties which make it an appealing molecule for incorporation; fluorescence and photodimerisation. The former is utilised to develop a single nucleotide polymorphism detection assay which is shown to allow for determination of the base present in a complementary strand of DNA. In addition to this, the photodimerisation properties of anthracene are used within a protein for the first time. This is utilised to develop a photoswitched binding protein, allowing for control of DNA binding of the protein. Further to this, the photodimerisation properties are utilised within oligonucleotides to achieve structural control of a G-quadruplex as well as photo-triggered release of single stranded DNA.

Type of Work:	Thesis (Doctorates > Ph.D.)
Award Type:	Doctorates > Ph.D.
Supervisor(s):	Supervisor(s) Email ORCID Peacock, Anna UNSPECIFIED UNSPECIFIED Tucker, James UNSPECIFIED UNSPECIFIED
Licence:	All rights reserved
College/Faculty:	Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department:	School of Chemistry
Funders:	None/not applicable
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH426 Genetics
URI:	http://etheses.bham.ac.uk/id/eprint/6369

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