Stem cell interactions with murine heart following ischaemia-reperfusion injury

Dhaliwal, Harpreet Kaur (2015). Stem cell interactions with murine heart following ischaemia-reperfusion injury. University of Birmingham. M.Res.

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Abstract

Haematopoietic stem cell (HSC) migration to injured cardiac tissue has shown to aid in cardiac tissue repair after acute myocardial infarction. Hesitation towards clinical trials however remain; due to the poor understanding of molecular mechanisms by which they are recruited to injured cardiac tissue. In this study the adhesive properties of HSCs to ischemia-reperfused (IR) cardiac tissue was investigated \(in\) \(vitro\). Following injury, adhesion of HSCs was significantly increased to cardiac tissue compared to sham controls. H\(_2\)O\(_2\) pre-treatment of HSCs did not enhance adhesion to both sham and injured cardiac tissue \(in\) \(vitro\). Blockade of CD31, CD44 and CD49d did not significantly attenuate HSC adhesion \(in\) \(vitro\). A change in adhesive properties of cardiac tissue after remote organ injury led to significantly increased adhesion of neutrophils \(in\) \(vitro\) to mice with liver injury induced by concanavalin A (ConA). This is the first study to show enhanced adhesion of neutrophils and HSCs to murine cardiac tissue in vitro following IR injury. If the mechanisms by which HSCs adhere to injured cardiac tissue can be identified and enhanced, it can help optimize the use of cellular therapy to reduce cardiac tissue damage after acute MI.

Type of Work:

Thesis (Masters by Research > M.Res.)

Award Type:

Masters by Research > M.Res.

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