Dialysis culture in animal cell growth and protein production

Amos, B. (1995). Dialysis culture in animal cell growth and protein production. University of Birmingham. Ph.D.

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Abstract

Hybridoma cells were grown in dialysis perfusion culture using a stirred reactor within which a tubular membrane was suspended. Nutrient and product flows occurred by diffusion processes alone, and were both to and from the culture environment A mathematical model of the transfer and reaction allowed prediction of steady state cell and metabolite concentrations. Steady states in cell concentration were observed for a range of perfusion rates and membrane areas. However the model could not be applied to predict steady state cell concentrations between changes in the medium. The perfusate consisted of basal medium only. Serum addition to the reactor itself resulted in decreased steady state cell densities except when it relieved a glucose limitation. Antibody was accumulated to high concentrations and yields on both basal medium and serum were many times those achieved in standard batch cultures. Cell viability fell to 30-50% but product quality did not appear to be adversely affected by the low viability. Recombinant CHO-320 cells also grew successfully under dialysis conditions and produced 7-interferon. Cell concentrations and viabilities were higher than those seen with the hybridoma. The insect cell line SF9 did not grow during dialysis perfusion, but post infection with a recombinant Baculovirus permitted the yield of \(\beta\)-galactosidase to double in dialysis culture.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

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