Dolea, Razvan Mihai (2014). The role of the NF-ΚB2 pathway in inflammatory bowel disease. University of Birmingham. M.Sc.
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Dolea14MSc.pdf
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Abstract
Perturbed NF-κB activity has been indentified in vitro and in vivo, as a potential factor in IBD and there is a lack of knowledge with regards to the role played by the alternative NF-κB pathway in the events leading to IBD.
Our experiments show that Nfκb2−/− mice are resistant to acute, DSS induced colitis when compared to Wt mice placed on the same procedure. Characterization of cellular infiltrates in the mesenteric lymph nodes of the Nfκb2−/− mice before and after the DSS procedure, showed a higher population of non-reactionary CD3+CD4+IL17+FOXP3+ cells. Splenic studies revealed the Nfκb2−/−mice display a reactionary B10 B cell population despite the mice suffering from a reduced total number of B cells. Nfκb2−/− Rag1−/− ,CD3ε−/− and RelB−/− mice placed on DSS treatment were found to be equally susceptible when compared to their Wt counterparts. Results from our chronic models of colitis showed no significant differences between Nfκb2−/− mice and Wt littermate controls in terms of susceptibility to colitis.
Our studies showed that crossing p100−/− mice with LtβR−/− mice partially rescues the LtβR−/− phenotype. The cervical and mesenteric lymph nodes of these mice have identifiable expression of CXCL13, CCL21, Gp38 and CR1 as evidenced by immune-fluorescence microscopy.
Type of Work: | Thesis (Masters by Research > M.Sc.) | |||||||||
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Award Type: | Masters by Research > M.Sc. | |||||||||
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College/Faculty: | Colleges (2008 onwards) > College of Medical & Dental Sciences | |||||||||
School or Department: | School of Immunity and Infection | |||||||||
Funders: | None/not applicable | |||||||||
Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) | |||||||||
URI: | http://etheses.bham.ac.uk/id/eprint/4895 |
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