Project 1: Characterization of EvgAS - two component signal transduction system in Escherichia coli and Project 2: Molecular mechanisms of lipid biosynthesis and transport in mycobacteria

Pathak, Prachi P. (2014). Project 1: Characterization of EvgAS - two component signal transduction system in Escherichia coli and Project 2: Molecular mechanisms of lipid biosynthesis and transport in mycobacteria. University of Birmingham. M.Res.

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Abstract

Project 1: Escherichia coli encounter many stresses, during passage through mammalian gut, including acid stress. There are three “Two component systems” (TCS) involved in AR2 network which responds to acid including EvgAS. Till today, the target genes under the control of EvgAS are poorly characterized. In our study, we have generated mutants and mapped them in gene encoding EvgS. Using LacZ assay and acid resistance assay we characterized their contribution to acid resistance in E.coli K12.

Project 2: Most of the antitTB drugs target the cell wall containing mycolyl-arabinogalactan-peptidoglycan complex which is essential in mycobacterial sp such as M. tuberculosis. Mycolic acids are essential components of the mycobacterial cell wall. In this study, we confirmed that gene encoding permease MSMEG4721, can be deleted in M. smegmatis without affecting cell viability. We have identified and characterized this mutant along with it’s ortholog of M. tuberculosis Rv2508, which is found to be involved in the process of mycolic acid reduction. The findings in this study not only help to understand complexities of mycobacterial cell wall biosynthesis but also denote potential new drug target for tuberculosis.

Type of Work:

Thesis (Masters by Research > M.Res.)

Award Type:

Masters by Research > M.Res.

Supervisor(s):