Interactions of 45s5 bioactive glass with the dentine-pulp complex

Comar, Eisha (2014). Interactions of 45s5 bioactive glass with the dentine-pulp complex. University of Birmingham. Ph.D.

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Abstract

The bioactive glass, 45S5, has been proposed for the treatment of dentine hypersensitivity due to its ability to physiochemically occlude dentinal tubules. However, its biological effects on the dentine-pulp complex require further elucidation. The aims of this study were to characterise the ionic dissolution products of bioactive glass and observe their effects on both pulpal cells and the dentine extracellular matrix. The ability of bioactive glass to release dentine matrix components (DMCs) in the presence of a physiological fluid, saline, was investigated. The bioactive glass-released DMCs were characterised biochemically and compared with those released by EDTA, a well-established extractant, using high-throughput proteomic techniques. Results revealed the differential solubilisation of a broad range of proteins, including growth factors and other bioactive molecules, many of which had not previously been identified in dentine. In vitro analysis of dental pulp cells exposed to DMCs released by bioactive glass in saline solution demonstrated a dose-dependent increase in cell proliferation, whilst the direct effects of the ionic dissolution products of bioactive glass were also stimulatory to OD-21 cells. The positive responses of cells to bioactive glass-released DMCs and its ionic dissolution products indicates exciting potential to harness bioactive approaches therapeutically for dentine tissue repair and regeneration.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

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