Gold nanoparticles: synthesis, characterisation and their effect on Pseudomonas flourescens

Nur, Yusuf (2013). Gold nanoparticles: synthesis, characterisation and their effect on Pseudomonas flourescens. University of Birmingham. Ph.D.

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Abstract

Both the fate and behaviour of the nanomaterials in the environment and their effects on the living organisms need to be better understood in order to maintain environmental health and ensure the sustainability of the important nanotechnology industry.

This dissertation focused on the effects of gold nanoparticles on the Pseudomonas fluorescens bacteria, as a model of the planktonic bacterial biomass.

Citrate capped and PVP Capped gold NPs were synthesized and characterised by measuring their relevant physicochemical properties such as size, shape, zeta potential, surface charge and stability in environmentally relevant ionic strength and in bacterial growth media.

The citrate capped AuNPs of (14 nm core size) had no effect on the bacterial growth as no inhibition was recorded when compared with control samples, while similar concentration and comparable sizes of PVP capped gold NPs have affected the bacterial growth as manifested through the reduction of optical density and demonstrated by the TEM images in the form of membrane damage including blebbing formation, tubular structures on the surface of the membrane of the bacterial cells and, in severe cases, the complete bursting of bacterial cells. It was found out that gold ions inhibit completely the bacterial growth as shown by optical density measurements.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):