Manzanares, Chloé (2013). Genetics of self-incompatibility in perennial ryegrass (Lolium perenne L.). University of Birmingham. Ph.D.
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Manzanares13PhD.pdf
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Abstract
Self-incompatibility (SI) prevents pollination by “self” pollen and promotes outbreeding. SI is a widespread mechanism among angiosperms. Grass SI is known to be controlled by two loci, S and Z, mapped in linkage group 1 and 2 respectively but the mechanism remains elusive.
SI in perennial ryegrass (Lolium perenne L.) is studied at the gene level for the S-locus. Using a fine-mapping approach, followed by the sequencing of the genomic S-locus region and the transcripts from pollen and stigma, the project identified four stigma S-candidate genes and potentially six pollen S-candidate genes. Moreover, using closely linked markers, the allelic diversity in a breeding population was assessed for both S and Z. This assay demonstrated that allelic diversity is maintained in a half-sib family recurrent selection programme and that even if the SI genes are unknown, it is possible to predict their genotypes.
Finally, self-compatibility has been reported in many grasses. Using self-compatible perennial ryegrass populations, two additional loci, F and T, have been investigated, by a mapping approach as well as observation of self-pollination under the microscope. For both loci, a distortion segregation was observed on linkage group 3 (F) and 5 (T) and initial marker recombination maps were created.
Type of Work: | Thesis (Doctorates > Ph.D.) | ||||||||||||
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Award Type: | Doctorates > Ph.D. | ||||||||||||
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College/Faculty: | Colleges (2008 onwards) > College of Life & Environmental Sciences | ||||||||||||
School or Department: | School of Biosciences | ||||||||||||
Funders: | Other | ||||||||||||
Other Funders: | Teagasc – the Agriculture and Food Development Authority, Ireland | ||||||||||||
Subjects: | Q Science > QK Botany | ||||||||||||
URI: | http://etheses.bham.ac.uk/id/eprint/4280 |
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