An investigation of the microdialysis technique to monitor metabolism in healthy and burned human skin

Breuning, Eleonore Elisabeth (2013). An investigation of the microdialysis technique to monitor metabolism in healthy and burned human skin. University of Birmingham. M.D.

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Abstract

Microdialysis is a method of monitoring biochemistry of tissues in-vivo, and is established as a clinical and research tool for monitoring of other tissue such as liver and brain. The aim of this thesis was to investigate the use of the microdialysis method in monitoring the human dermis in healthy and burned skin, with a view to its use as a tool to detect progression of the burn wound. Ten healthy volunteers were used to establish a normal range for glucose, lactate, pyruvate and glycerol levels, and to assess the effect of temperature on results. Ten patients with burns <15%TBSA were recruited and microdialysis was undertaken in zones of coagulation and stasis and in unburned skin in each patient from recruitment until36 hours post-bum. A further 2 patients with burns >30%TBSA were also studied. Biochemical findings and the result of amino acid assays are reported. An attempt at correlating biochemical findings with Laser Doppler Images was made, to establish the role of the technique in predicting wound progression. However no wounds showed any evidence of progression in the areas of probe placement over the time of the study.

Type of Work:

Thesis (Higher Doctorates > M.D.)

Award Type:

Higher Doctorates > M.D.

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