Transcriptomics analysis of phloem-feeding insect resistance in rice germplasm

Ab-Ghaffar, Mohamad Bahagia (2012). Transcriptomics analysis of phloem-feeding insect resistance in rice germplasm. University of Birmingham. Ph.D.

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Abstract

The Brown Plant Hopper (BPH) is a serious pest of rice in Asia. Development of novel control strategies can be facilitated by a comparison of BPH feeding behaviour on varieties exhibiting natural genetic variation, and then an elucidation of the underlying mechanisms of resistance. We began by understanding BPH feeding behaviour on 12 rice varieties with different resistance background using Electrical Penetration Graph (EPG) and honeydew clock experiments. Seven feeding behaviours (waveforms) were identified and could be classified into two phases, feeding and non- feeding. Cluster analysis has separated the 12 varieties into 3 main groups, resistant, moderate and susceptible. Then, we undertook microarray analysis on all varieties to identify candidate genes which may contribute to resistance. The results reveal the difference between resistant and susceptible varieties. The data agree with EPG and honeydew clock experiments. A total of 21556 probes passed filter in statistical analysis using quantile method (in Genespring) and 239 probes significantly contributed to the difference between resistant versus susceptible (Volcano analysis). Some of them were found to be highly correlated with EPG data and could therefore be potential resistance candidate genes against BPH such as gene encoding hexose transporter, protein kinases, Alpha-DOX2 and peroxidases

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

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