The use of proteomics techniques to identify potential markers of early stage colorectal cancer

O'Neil, Donna (2011). The use of proteomics techniques to identify potential markers of early stage colorectal cancer. University of Birmingham. Ph.D.

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Abstract

Colorectal cancer (CRC) is the third most common cancer and the second most common cause of cancer related deaths in the UK. Research has shown that the five year survival rate for patients if diagnosed at an early stage is 83% however, only 11% of cases are diagnosed at this stage. The aim of this study was to use proteomic approaches to investigate secreted proteins from colorectal cancer cell lines to identify candidate biomarkers for early stage diagnosis. Microvesicles (MVs) are a mixed population of vesicles that are released by a wide range of cells and are thought to play a role in tumour development and progression. Stable Isotope Labeling of Amino Acids in Culture (SILAC) was used to investigate the relative abundance of proteins secreted in MVs released by two cell lines that are used as a model of early tumour progression. This study identified 86 potential candidates that demonstrated increased release and six of these proteins (AGR2, OLFM4, SBP1, HSP90α, HSP90β and CEACAM5) were selected for further investigation by Western blot analysis. These proteins show potential as markers of early stage CRC and would be suitable for further validation in patient serum samples.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):