Optimization of the matched irrigation to obturation with hydraulic cement sealers strategy

Bhandari, Sweta Surana ORCID: 0000-0002-5886-8035 (2025). Optimization of the matched irrigation to obturation with hydraulic cement sealers strategy. University of Birmingham. M.Sc.

Bhandari2025MScByRes.pdf Text - Accepted Version Available under License All rights reserved. Download (8MB)

Abstract

Tricalcium silicate sealer materials are vastly used in root canal obturation, mainly due to the calcium hydroxide release which is responsible for their biological and antimicrobial activity. The current study discusses the importance of selecting appropriate irrigation protocols for dentine preparation during root canal treatment for the choice of sealer and improve the adhesion and sealing ability of sealers with dentine. The study investigated the effect of different irrigation techniques, including continuous chelation, on dentine microstructure and aimed to choose the best irrigation protocol for endodontic treatment using Hydraulic calcium silicate cement (HCSC) sealers for obturation. The research also explored changes in the antimicrobial properties of HCSC sealers on interaction with dentine after irrigation with different chemical solutions using a split tooth model. Sterilisation of sample materials were also checked during the project.
Irrigating solutions can cause structural and chemical alterations of root dentin, affecting the mechanical and chemical adhesion of sealers to dentin and the antimicrobial properties of both dentin and sealer. Sodium hypochlorite (NaOCl), most widely used irrigant, being antimicrobial, eliminates the microbial load but can cause detrimental effects on the collagen. The most employed calcium chelator, Ethylenediaminetetraacetic acid (EDTA) can dissolve the inorganic debris and clear the smear layer produced by instrumentation but reduces the root dentine microhardness due to demineralization of the hydroxyapatite components of dentin. Hydraulic calcium silicate sealers interact with dentin more chemically and do not benefit from the property of calcium chelation and smear layer removal. The study used BioRoot RCS, TotalFill BC, and AH Plus jet as the test materials, and NaOCl, EDTA & Hydroxy ethylidene Di phosphonic Acid (HEDP) as the irrigation solutions. The results showed that in comparison to EDTA, irrigation with
HEDP resulted in a larger mineral concentration zone at the interface for hydraulic cements with less erosion of dentine. Furthermore, continuous chelation with HEDP improved the antimicrobial properties of the HCSC sealers without erosion of dentine making it the best irrigation protocol for endodontic treatment using tricalcium silicate sealers for obturation. Another finding of this project highlighted that hydraulic cement sealers are impacted by sterilization processes used prior to biological testing.
Regarding the method for studying the dentine sealer interface this study also concluded that when employing hydraulic cements, scanning electron microscopy and confocal laser scanning microscopy are not acceptable methods for studying the interface, Fourier-transform infrared spectroscopy can be used instead.

Type of Work:	Thesis (Masters by Research > M.Sc.)
Award Type:	Masters by Research > M.Sc.
Supervisor(s):	Supervisor(s) Email ORCID Camilleri, Josette UNSPECIFIED orcid.org/0000-0003-3556-6365 Palin, William UNSPECIFIED orcid.org/0000-0001-6790-8433 Kuehne, Sarah UNSPECIFIED orcid.org/0000-0001-6790-8433
Licence:	All rights reserved
College/Faculty:	Colleges (former) > College of Medical & Dental Sciences
School or Department:	School of Dentistry
Funders:	None/not applicable
Subjects:	R Medicine > RK Dentistry
URI:	http://etheses.bham.ac.uk/id/eprint/15724

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