Interaction of turnip mosaic virus (TUmV) with members of the brassicaceae

Hughes, Sara Louise (2002). Interaction of turnip mosaic virus (TUmV) with members of the brassicaceae. University of Birmingham. Ph.D.

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Abstract

Attempts to identify Turnip mosaic virus (TuMV) resistance in ecotypes of Arabidopsis thaliana proved unsuccessful, although methods for successful inoculation and assessment of TuMV infection were developed. Thirty-three sources of resistance to TuMV have been identified in Brassica rapa and B. napus. Fourteen lines were resistant to TuMV isolates representing the most prevalent TuMV pathotypes, 1, 3 and 4. Lines with novel spécificités were also identified. For the first time, a source of resistance to pathotype 4 was identified in B. napus line 22S. The genetic basis of resistance to TuMV was determined for seven Brassica lines. For six lines, crosses were made to susceptible genotypes and segregation of the resistance phenotype was followed in the backcross progeny. For the remaining line, Jong Bai No. 1, segregation of the resistance phenotype was followed in an F2 population. Monogenic, dominant resistance models were proposed for B. napuslines 22S, 165 and B. rapa line Jong Bai No. 2. Recessive monogenic and multigenic models were proposed for the remaining B. rapa lines. The allele conferring novel resistance in line 22S, TuRB03, was mapped to the lower region of B. napus linkage group N6, using amplified fragment length polymorphism and microsatellite bulk segregant analysis, to the same interval as TuRBOl and TuRBOlb. The allele conferring novel resistance in line Jong Bai No. 2 was roughly mapped to the upper region of linkage group N6. The alleles conferring resistance to pathotypes 1 and 3 in line 165 were loosely mapped to N6 and N8 (or N18) respectively.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):