The Role of the Free Fatty Acid Receptor 4 (FFAR4)/GPR120 in Adipocyte Lipolysis

Tripp, Emma (2023). The Role of the Free Fatty Acid Receptor 4 (FFAR4)/GPR120 in Adipocyte Lipolysis. University of Birmingham. Ph.D.

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Abstract

Metabolites are intermediates or end products of metabolism. They are also building blocks for a variety of metabolic pathways and often function as intracellular and extracellular signalling molecules. Several G protein-coupled receptors (GPCRs) have the capacity to sense metabolites to control hormone secretion, or to regulate the metabolic activity of certain cell types. In metabolic diseases, such as diabetes and obesity, metabolites are dysregulated. Therefore, further understanding the mechanisms behind metabolite sensing GPCR signalling could help to identify novel therapeutic targets. Within this study, using bioluminescence resonance energy transfer (BRET) and other imaging-based approaches, I present evidence that the free fatty acid receptor 4 (FFAR4), a GPCR for long chain fatty acids (LCFA), is localised in close proximity to the lipid droplet membrane in adipocytes, where it can act as an intracrine negative-feedback regulator of lipolysis by inhibiting cyclic adenosine monophosphate (cAMP) production in response to the local concentration of intracellular FFAs. This intracrine signalling phenomenon is anticipated to assist the FFAR4 in its control of adipocyte metabolism.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):