The development of multimodal nanoprobes for cell tracking in the treatment of autoimmune hepatitis

Chauhan, Tasha (2022). The development of multimodal nanoprobes for cell tracking in the treatment of autoimmune hepatitis. University of Birmingham. Ph.D.

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Abstract

The ability to track cells both in model systems and in the body is needed to enable scientists to better develop and understand new cell therapies and construct a wider understanding of the roles of different cell engagements. To explore this, in this thesis, cells are labelled with multimodal nanoprobes and a range of imaging modalities used for tracking and detection. Gold nanoparticles successfully performed as detectable scaffolds, holding both the coating and the luminescent compound, IrS1 (Iridium(III) bis(phenylquinoline-C2,N’) 5,5’-bis(mercaptomethyl)- 2,2’-bipyridine), and were able to enter human lymphocytes to allow detection utilising their optical and luminescent properties. Gold nanoparticles ranging in sizes of 13, 25, 50 and 100nm with different stabilising agents were compared for their loading abilities and biocompatibility. It was found the smaller particles with smaller- chain stabilising agents had minimal impact on the phenotypical and functional behaviour of the immune cells and were more readily taken up by the cells. Regulatory T cells dosed with nanoprobes with a gold core of 25nm coated with Zonyl and IrS1 were administered to human liver sections which were then imaged using Micro-CT and Multiphoton microscopy to observe the localisation of the transplanted cells.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

Supervisor(s):