Understanding gene and protein function of MYB93 in tomato and Arabidopsis

Cao, Xulyu (2022). Understanding gene and protein function of MYB93 in tomato and Arabidopsis. University of Birmingham. Ph.D.

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Abstract

AtMYB93 functions as a negative regulator of lateral root development in Arabidopsis. The focus of this study is to identify MYB93 orthologues in tomato and study their function. I have identified three SlMYB93s in tomato, which are SlMYB93-1, SlMYB93-2 and SlMYB93-3.
Overexpress of SlMYB93-1 and SlMYB93-2 in Arabidopsis exhibit reduced lateral root density, suggests that SlMYB93-1 and SlMYB93-2 have similar function on lateral root development with AtMYB93 in Arabidopsis

By analysing RNAseq data of Atmyb93 mutant, I have identified 255 differentially expressed genes, which function in various aspects plant growth, such as sugar transport, flavonoid metabolism, cell wall development. Further analysis has identified AtGH9C3 and AtCASPL1D2 have AtMYB93 binding motifs in their promoter regions., which makes them likely to be direct target genes of AtMYB93. It is hypothesised that AtMYB93 controls lateral root development by regulating AtGH9C3 and AtCASPL1D2 expression. Interestingly, AtMYB93 is upregulated in response to deficiency, and S deficiency also inhibits lateral root development in Arabidopsis. AtMYB93 is potentially identified as a lateral root inhibitor in response to S deficiency.

Type of Work:

Thesis (Doctorates > Ph.D.)

Award Type:

Doctorates > Ph.D.

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