Point of care diagnostics for traumatic brain injury

Banbury, Carl (2021). Point of care diagnostics for traumatic brain injury. University of Birmingham. Ph.D.

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Abstract

Traumatic brain injury (TBI) is a major burden on healthcare services worldwide, which currently lacks an effective method for point-­of­-care (PoC) diagnostics and monitoring. In this thesis, the prospect of using Raman spectroscopy, as a non­invasive means to mea­sure chemical changes indicative of TBI from the back of the eye is explored. Through the development of multivariate analysis methods, self­ organising maps are highlighted as a superior alternative to principal component analysis and are successfully used to in­troduce a new means of classification. A classification accuracy of > 93 % is demonstrated over five tissue types applied to anatomical layers of ocular porcine eyes. Subsequently, in a clinically relevant murine model of TBI, an ability to accurately identify TBI and in­jury severity from Raman spectra of the retina is demonstrated for the first time. Using feature extraction, intrinsically linked to the classification result, the findings are associ­ated with a decrease in cardiolipin linked to metabolic distress, which is a hallmark of TBI. Finally, in an effort to translate the technology, a (patent pending) handheld system has been developed. Simultaneously, fundus photography is combined with eye safe Raman spectroscopy, measuring high wavenumber bands from a three­dimensional printed tissue phantom of the undilated human eye.

Type of Work: Thesis (Doctorates > Ph.D.)
Award Type: Doctorates > Ph.D.
Supervisor(s):
Supervisor(s)EmailORCID
Goldberg Oppenheimer, PolaUNSPECIFIEDUNSPECIFIED
Styles, Iain BUNSPECIFIEDUNSPECIFIED
Eisenstein, NeilUNSPECIFIEDUNSPECIFIED
Belli, AntonioUNSPECIFIEDUNSPECIFIED
Logan, AnnUNSPECIFIEDUNSPECIFIED
Licence: All rights reserved
College/Faculty: Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department: School of Chemical Engineering
Funders: Engineering and Physical Sciences Research Council
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QC Physics
Q Science > QD Chemistry
R Medicine > RE Ophthalmology
URI: http://etheses.bham.ac.uk/id/eprint/11353

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