Point of care diagnostics for traumatic brain injury

Banbury, Carl (2021). Point of care diagnostics for traumatic brain injury. University of Birmingham. Ph.D.

Preview

Banbury2021PhD.pdf Text - Accepted Version Available under License All rights reserved. Download (27MB) | Preview

Abstract

Traumatic brain injury (TBI) is a major burden on healthcare services worldwide, which currently lacks an effective method for point-­of­-care (PoC) diagnostics and monitoring. In this thesis, the prospect of using Raman spectroscopy, as a non­invasive means to mea­sure chemical changes indicative of TBI from the back of the eye is explored. Through the development of multivariate analysis methods, self­ organising maps are highlighted as a superior alternative to principal component analysis and are successfully used to in­troduce a new means of classification. A classification accuracy of > 93 % is demonstrated over five tissue types applied to anatomical layers of ocular porcine eyes. Subsequently, in a clinically relevant murine model of TBI, an ability to accurately identify TBI and in­jury severity from Raman spectra of the retina is demonstrated for the first time. Using feature extraction, intrinsically linked to the classification result, the findings are associ­ated with a decrease in cardiolipin linked to metabolic distress, which is a hallmark of TBI. Finally, in an effort to translate the technology, a (patent pending) handheld system has been developed. Simultaneously, fundus photography is combined with eye safe Raman spectroscopy, measuring high wavenumber bands from a three­dimensional printed tissue phantom of the undilated human eye.

Type of Work:	Thesis (Doctorates > Ph.D.)
Award Type:	Doctorates > Ph.D.
Supervisor(s):	Supervisor(s) Email ORCID Goldberg Oppenheimer, Pola UNSPECIFIED UNSPECIFIED Styles, Iain B UNSPECIFIED UNSPECIFIED Eisenstein, Neil UNSPECIFIED UNSPECIFIED Belli, Antonio UNSPECIFIED UNSPECIFIED Logan, Ann UNSPECIFIED UNSPECIFIED
Licence:	All rights reserved
College/Faculty:	Colleges (2008 onwards) > College of Engineering & Physical Sciences
School or Department:	School of Chemical Engineering
Funders:	Engineering and Physical Sciences Research Council
Subjects:	Q Science > QA Mathematics > QA75 Electronic computers. Computer science Q Science > QC Physics Q Science > QD Chemistry R Medicine > RE Ophthalmology
URI:	http://etheses.bham.ac.uk/id/eprint/11353

Actions

	Request a Correction
	View Item

Downloads