O'Shea, Christopher 
ORCID: 0000-0003-3030-7364
(2020).
Development and application of novel processing tools and methods for cardiac optical mapping.
University of Birmingham.
Ph.D.
|
OShea2020PhD.pdf
Text - Accepted Version Available under License All rights reserved. Download (5MB) | Preview |
Abstract
Cardiac optical mapping provides unparalleled spatio-temporal resolution information of cardiac electrophysiology. It has hence emerged as an important technology in understanding cardiac electrical behaviour in physiological and pathophysiological states. There is a requirement for effective data analysis tools that are high-throughput, robustly characterised and flexible with regards to a growing array of experimental models.
In this thesis a MATLAB based software, ElectroMap, was developed for analysis of diverse optical mapping datasets. ElectroMap incorporates existing and novel methods to allow quantification and mapping of action potential and calcium transient morphology and activation/repolarisation times. Automated pacing cycle length detection and segmentation were implemented, realising high-throughput analysis of beat-to-beat responses and transient behaviour. Standalone modules dedicated to calculation of conduction velocity and alternans were introduced, allowing thorough integration of key factors in arrhythmogenesis. Semi-automated analysis of temporal variations in wave morphology were developed from previous methodologies for electrogram analysis. Algorithms to use fractional rate of change of fluorescence as a measure of conduction were also introduced to the software. Algorithms were tested in silico datasets, mouse and guinea pig optical mapping datasets and preliminary experiments also showed use for in vivo human electrogram mapping of atrial fibrillation.
| Type of Work: | Thesis (Doctorates > Ph.D.) | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Award Type: | Doctorates > Ph.D. | ||||||||||||||||||
| Supervisor(s): |
|
||||||||||||||||||
| Licence: | All rights reserved | ||||||||||||||||||
| College/Faculty: | Colleges (2008 onwards) > College of Engineering & Physical Sciences | ||||||||||||||||||
| School or Department: | School of Chemistry | ||||||||||||||||||
| Funders: | Engineering and Physical Sciences Research Council | ||||||||||||||||||
| Subjects: | Q Science > QP Physiology | ||||||||||||||||||
| URI: | http://etheses.bham.ac.uk/id/eprint/10190 |
Actions
 |
Request a Correction |
 |
View Item |
Downloads
Downloads per month over past year